High Performance Liquid Chromatography and Mass Spectrometry

High-performance liquid chromatography (HPLC), also known as high-pressure liquid chromatography, is an advanced type of LC. HPLC is amenable to a wide range of applications, such as pharmaceuticals and food analysis. It is especially useful for low or non-volatile organic compounds, which cannot be handled with gas chromatography. Liquid chromatography–mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). While liquid chromatography separates mixtures with multiple components, mass spectrometry provides structural identity of the individual components with high molecular specificity and detection sensitivity. Therefore, LC-MS may be applied in a wide range of sectors including biotechnology, environment monitoring, food processing, and pharmaceutical, agrochemical, and cosmetic industries. The book has primarily been designed for analysts who are unfamiliar with this technique or have limited training and experience in this or have constraints in finding a suitable HPLC method. The book presents a comprehensive compilation of reviews, opinions and applications of the use of Mass Spectrometry to analyze biomolecules.